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vector plh sgrna1 boxb ms2 pp7  (Addgene inc)


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    Addgene inc vector plh sgrna1 boxb ms2 pp7
    Vector Plh Sgrna1 Boxb Ms2 Pp7, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plh+sgrna1+ms2+pp7/pLH-sgRNA1-boxB-MS2-PP7+(Plasmid+%2375397)/pmc12423276-177-12-14
    Average 93 stars, based on 1 article reviews
    vector plh sgrna1 boxb ms2 pp7 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    CRISPR:

    Article Title: Real-Time Imaging of Specific Genomic Loci With CRISPR/dCas9 in Human Cells Using CRISPRainbow
    Article Snippet: Ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich, catalog number: E9884), storage RT B. Cloning annealed oligos into vectors 1. .. CRISPR sgRNA vectors with stem loops: pLH-sgRNA1-2XMS2 (Addgene, ID: 75389), pLH-sgRNA1-2XPP7 (Addgene, ID: 75390), pLH-sgRNA1-2XboxB (Addgene, ID: 75391), pLH-sgRNA1-MS2-PP7 (Addgene, ID: 75392), pLH-sgRNA1-PP7-boxB (Addgene, ID: 75393), pLH-sgRNA1-boxB-MS2 (Addgene, ID: 75396), and pLH-sgRNA1-boxB-MS2-PP7 (Addgene, ID: 75397), storage: -20 °C 2. .. ATP, 100 mM (New England Biolabs, catalog number: P0756S), storage: -20 °C 3.

    Cloning:

    Article Title: Engineering inducible biomolecular assemblies for genome imaging and manipulation in living cells
    Article Snippet: .. For the assembly of each sgRNA in to pLH-sgRNA1-MS2-PP7 (Addgene #75392), the Golden Gate cloning method was used for replacing CcdB gene with each guide RNA sequence between two BbsI sites by following the protocol from the previous publication . .. Briefly, annealing of sgRNA oligos was done using the following reaction mix and thermocycle: 40 μl annealing buffer (100 mM Tris-HCl pH8.0, 50 mM NaCl, 1 mM EDTA), 5 μl 100 μM Forward oligo, 5 μl 100 μM Reverse oligo.

    Sequencing:

    Article Title: Engineering inducible biomolecular assemblies for genome imaging and manipulation in living cells
    Article Snippet: .. For the assembly of each sgRNA in to pLH-sgRNA1-MS2-PP7 (Addgene #75392), the Golden Gate cloning method was used for replacing CcdB gene with each guide RNA sequence between two BbsI sites by following the protocol from the previous publication . .. Briefly, annealing of sgRNA oligos was done using the following reaction mix and thermocycle: 40 μl annealing buffer (100 mM Tris-HCl pH8.0, 50 mM NaCl, 1 mM EDTA), 5 μl 100 μM Forward oligo, 5 μl 100 μM Reverse oligo.

    Construct:

    Article Title: Engineering inducible biomolecular assemblies for genome imaging and manipulation in living cells
    Article Snippet: N-Venus and C-Venus were inserted into pHAGE-EFS-MCP-3XBFP-NLS (Addgene #75384) and pHAGE-EFS-PCP-3XGFP-NLS (Addgene#75385) in between BamHI and XhoI to replace 3xBFP and 3xGFP, respectively. .. The gRNA constructs with MS2-PP7 loops were generated based on pLH-sgRNA1-MS2-PP7 (Addgene #75392). ..

    Generated:

    Article Title: Engineering inducible biomolecular assemblies for genome imaging and manipulation in living cells
    Article Snippet: N-Venus and C-Venus were inserted into pHAGE-EFS-MCP-3XBFP-NLS (Addgene #75384) and pHAGE-EFS-PCP-3XGFP-NLS (Addgene#75385) in between BamHI and XhoI to replace 3xBFP and 3xGFP, respectively. .. The gRNA constructs with MS2-PP7 loops were generated based on pLH-sgRNA1-MS2-PP7 (Addgene #75392). ..



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